胰高血糖素样肽-2和二肽基肽酶Ⅳ抑制剂联合使用对断奶仔猪肠上皮细胞的影响

doi:10.11843/j.issn.0366-6964.2017.01.012

Abstract

Abstract:

This study was conducted to investigate the effects of the combination of Glucagon-like peptide-2 (GLP-2) and dipeptidyl peptidase-Ⅳ (DPP-Ⅳ) inhibitors with different concentrations on cell proliferation, metabolism and enzyme activity of primarily intestinal epithelial cells cultured in vitro from 28-day-old weaned piglets, and aimed to explore the method of promoting GLP-2 effects. The experiment, taking 28-day-old weaned piglets’ intestinal epithelial cells as a model, studied the effects of DPP-Ⅳ inhibitors (KR62436) with different concentrations on the cell proliferation and metabolism. The 2×3 factorial design was adopted to study the effects of combination of GLP-2 (1×10^-10, 1×10^-9 and 1×10^-8 mol•L^-1) and KR62436 (0 and 1×10^-11 mol•L^-1) on cell proliferation, metabolism and apoptosis. The results were as follows: there was no significant effect of DPP-Ⅳ with 1×10^-11 mol•L^-1 on cell number, MTT OD, cell protein retention, total cell protein level, LDH activity, CK activity and Na⁺, K⁺-ATPase activity (P>0.05). Compared with the control group, the DPP-Ⅳ group with 1×10^-10 mol•L^-1 showed lower MTT OD value (P<0.05), and higher LDH and CK activity (P<0.05). In the groups that treated with different concentrations of GLP-2 and low concentrations of KR62436, with the GLP-2 doses increasing, cell number, MTT OD value, cell protein retention, total cell protein level and Na⁺, K⁺-ATPase activity increased significantly (P<0.05), and LDH and CK activity decreased significantly (P<0.05). KR62436 could significantly promote GLP-2 effects. The results indicate that the low concentration DPP-Ⅳ inhibitors have no significant effect on cell growth, while the high concentration DPP-Ⅳ inhibitor have a negative effect on cell metabolism and integrity. Combination of GLP-2 and low concentrations DPP-Ⅳ inhibitors may have favorable effects on intestinal epithelial cell proliferation, metabolism and integrity than GLP-2 alone.

CLC Number:

S828
S811.2

JIA Gang, DENG Qiu-hong, JIANG Rong-chuan, ZHAO Hua, CHEN Xiao-ling, LIU Guang-mang, WANG Kang-ning. Effects of the Combination of Glucagon-like Peptide-2 and Dipeptidyl Peptidase-Ⅳ Inhibitors on Intestinal Epithelial Cells of Weaned Piglets in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 99-107.

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Effects of the Combination of Glucagon-like Peptide-2 and Dipeptidyl Peptidase-Ⅳ Inhibitors on Intestinal Epithelial Cells of Weaned Piglets in vitro

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